12-Hydroxy-4,8,10-heptadecatrienoic acid (12-HHT) is a bioactive lipid mediator derived from thromboxane A2 hydrolysis, widely used in biochemical research and pharmaceutical development. It serves as a key research tool for studying platelet activation, inflammation, and vascular biology. In cell biology, 12-HHT acts as an endogenous ligand for the G-protein coupled receptor BLT2, regulating cell migration, proliferation, and survival in various cell types. It is extensively employed in in vitro assays to investigate BLT2-mediated signaling pathways and their roles in cancer progression, particularly in breast and prostate cancer research. Additionally, 12-HHT is used in the development of anti-inflammatory and anti-thrombotic drugs, as well as in the study of skin barrier function and wound healing processes. Its high purity and stability make it an essential reagent for lipidomics and signal transduction research.

GHS Classification: H315 (Causes skin irritation), H319 (Causes serious eye irritation), H335 (May cause respiratory irritation). Signal Word: Warning. Acute oral LD50 >2000 mg/kg (low toxicity). Skin contact may cause redness, itching, or mild dermatitis; wear nitrile gloves and protective clothing when handling. Eye contact can lead to severe irritation and conjunctivitis; use splash-proof goggles and rinse eyes with plenty of water for at least 15 minutes if exposed. Inhalation of mist or vapor may irritate the respiratory tract, causing cough or shortness of breath; use in a fume hood or well-ventilated area and wear a dust mask. No evidence of carcinogenicity, mutagenicity, or reproductive toxicity. Harmful to aquatic life; prevent discharge into waterways. Store separately from strong oxidants and acids; neutralize spills with inert absorbent material and dispose of according to local hazardous waste regulations.