Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
1. Basic Product Profile
- Full IUPAC Name: tetrasodium 1,3,6,8-pyrenetetrasulfonate
- Aliases: PTS, Pyrene tetrasulfonic acid tetrasodium salt, HPTS, Pyranine
- CAS Number: 59572-10-0
- EINECS: N/A
- Molecular Formula: C16H4Na4O12S4
- Molecular Weight: 610.41
- Chemical Classification: Water-soluble polycyclic aromatic fluorescent dye, pH fluorescent probe, biological staining reagent, industrial fluorescent tracer
- Core Advantages
Highly water-soluble anionic pyrene derivative with four sulfonate groups, excellent fluorescence quantum yield. Strong pH responsiveness in aqueous media; stable in wide pH range, low cell toxicity, no permeation through cell membranes. Widely used as ratiometric pH sensor for intracellular/extracellular fluid testing, fluorescent tracer for water flow detection, fluorescent stain for polymer and biological research, and standard fluorescent reference material for analytical laboratories.
2. Physical & Chemical Properties
2.1 Physical Index
- Appearance: Bright yellow to orange crystalline powder
- Odor: Odorless
- Solubility: Freely soluble in water; slightly soluble in methanol; insoluble in ethanol, acetone, ether, aliphatic and aromatic non-polar solvents
- Melting characteristic: Decomposes above 300 °C without distinct melting point
- Fluorescence spectrum: Excitation λmax = 405 nm; dual emission peaks 430 nm (acid form) / 510 nm (base form)
- pH response range: pH 5–9 linear ratiometric fluorescence change
- Hygroscopy: Moderately hygroscopic under high humidity air
- Aqueous solution color: Yellow-green fluorescent solution under UV light
2.2 Chemical Properties
- Tetrasulfonate anionic structure: Four sodium sulfonate groups grafted on pyrene core, endowing complete water solubility
- pH-sensitive protonation: Central pyrene hydroxyl group undergoes reversible protonation, shifts fluorescence emission wavelength for ratiometric detection
- Photostability: Good light fastness compared to common fluorescent dyes; prolonged strong UV irradiation causes slight fluorescence attenuation
- Acid & Alkali Stability: Stable across pH 2–12; sulfonate groups do not hydrolyze under weak acid/alkali heating
- Thermal Stability: No decomposition below 200 °C; high temperature over 280 °C breaks pyrene conjugated skeleton
- Compatibility: Compatible with aqueous buffer solutions, anionic surfactants; incompatible with cationic polymers, cationic dyes, heavy metal ions (causes precipitation)
3. Main Application Fields
3.1 Biochemical & Cell Research (Core Usage)
- Ratiometric fluorescent pH probe for cytoplasm, lysosome and extracellular fluid pH measurement;
- Water-soluble fluorescent marker for liposome, microfluidic and membrane permeability experiments;
- Fluorescent tracer in cell culture medium with low cytotoxicity.
3.2 Environmental & Hydraulic Testing
Fluorescent tracing agent for river, groundwater, pipeline flow velocity and leakage detection; high water solubility ensures uniform dispersion.
3.3 Analytical Chemistry Standard
Calibration reference for fluorescence spectrophotometer, standard quantum yield reference substance for fluorescence instrument correction.
3.4 Polymer & Coating Auxiliary
Water-based fluorescent colorant for waterborne coatings, printing ink and water-soluble polymer fluorescent labeling.
4. Quality Specification Table
| Test Item | Standard Index |
| CAS No. | 59572-10-0 |
| Molecular Formula | C16H4Na4O12S4 |
| Molecular Weight | 610.41 |
| Appearance | Orange crystalline powder |
| HPLC Purity | Research Grade ≥99.0%; Industrial Tracer Grade ≥98.0% |
| Moisture Content (KF) | ≤0.50% |
| Free Sulfate Impurity | ≤0.10% |
| Single Unknown Impurity | ≤0.10% |
| Total Heavy Metals (Pb) | ≤10 ppm |
| Residue on Ignition | 38–42% |
| Bulk Package | 25kg aluminum foil lined fiber drum |
| Sample Package | 5g / 100g light-proof sealed plastic bottle |
| Storage Requirements | Hermetic light-proof dry warehouse ≤25℃; isolate direct sunlight, cationic chemicals and heavy metal salts |
| Transportation Rule | Non-hazardous fluorescent sulfonate salt; moisture-proof light-proof sealed packaging required during transit |
| Shelf Life | 36 months under intact light-proof sealed packaging |
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