Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
1. Basic Info
Name: β-Diphosphopyridine nucleotide
Alias: NAD, Nicotinamide adenine dinucleotide, NAD oxidized form
CAS No.: 53-84-9
Molecular Formula: C21H27N7O14P2
Molecular Weight: 663.43
2. Physical & Chemical Properties
Physical Properties
- Appearance: White or off-white crystalline powder, odorless
- Solubility: Freely soluble in water; slightly soluble in methanol; insoluble in ethanol, acetone, ether, alkane solvents
- Hygroscopy: Strongly hygroscopic, rapidly absorbs water vapor in humid air
- Aqueous solution pH: Weakly acidic; stable range pH 6.0–8.0
- UV absorption characteristic: Maximum absorption at 259 nm; after reduction to NADH, new absorption peak at 340 nm
- Thermal property: No clear melting point; gradually decomposes above 90℃
Chemical Properties
- Molecular structure: Consists of nicotinamide mononucleotide (NMN) and adenosine monophosphate linked by diphosphate bridge; contains nicotinamide redox active group
- Redox characteristic: Oxidized form NAD accepts hydrogen to generate reduced NADH; reversible redox reaction, core coenzyme for cellular respiration
- Hydrolysis susceptibility: Diphosphate bond easily hydrolyzed under strong acid or high temperature, decomposes into NMN and AMP; stable under neutral mild conditions
- Photodegradation: UV light accelerates decomposition, activity decreases obviously; must be stored away from light
- Compatibility: Compatible with neutral biological buffers; incompatible with strong oxidants, heavy metal ions, concentrated acid/alkali
- Enzymatic characteristic: Acts as coenzyme for dehydrogenase, participates in glycolysis, tricarboxylic acid cycle, fatty acid metabolism
3. Main Application Fields
1. Biochemical Laboratory Core Reagent (Core Application)
- Coenzyme substrate for dehydrogenase enzyme activity detection (lactate dehydrogenase, alcohol dehydrogenase etc.)
- Standard reagent for cell metabolism, energy metabolism research
- Colorimetric / spectrophotometric assay raw material for biochemical detection kits
2. Cell Culture & Biomedical Research
- Raw material for in vitro cell energy metabolism model experiments
- Study on oxidative stress, mitochondrial function, aging-related mechanism research
3. Diagnostic Kit Raw Material
Clinical biochemical detection kit auxiliary raw material for blood lactic acid, triglyceride, glucose quantitative testing
4. Microbial Fermentation Research
Coenzyme additive for strain fermentation experiments to study metabolic pathway transformation
4. Quality Specification Table
| Test Item | Standard Index |
| CAS Number | 53-84-9 |
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight | 663.43 |
| Appearance | White crystalline powder |
| HPLC Purity | Biochemical Grade ≥98.0%; Reference Standard ≥99.0% |
| Moisture Content (KF) | ≤8.0% |
| Related Impurities | Total impurities ≤2.0% |
| Heavy Metals (Pb) | ≤10 ppm |
| Storage Condition | Hermetic vacuum, light-proof, -20℃ low temperature storage; avoid repeated freeze-thaw cycles |
| Transportation | Cold insulated sealed packaging, moisture-proof, light-proof |
| Shelf Life | 24 months under intact vacuum packaging at -20℃ |
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