Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
1. Basic Info
Name: Ethylenebis(oxyethylenenitrilo)tetraacetic acid
Alias: EGTA, Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid
CAS No.: 67-42-5
Molecular Formula: C14H24N2O10
Molecular Weight: 380.34
2. Physical & Chemical Properties
Physical Properties
- Appearance: White crystalline powder, odorless
- Solubility: Insoluble in water, ether; slightly soluble in cold alcohol, soluble in dilute alkaline aqueous solution, hot DMSO
- Melting Point: Decomposes above 240 ℃ without clear melting point
- Hygroscopy: Slightly hygroscopic under high humidity
- Crystal form: Fine crystalline powder after recrystallization from dilute alkali-acid precipitation
Chemical Properties
- Molecular structure: Contains 2 amino nitrogen atoms, 4 carboxyl groups and two ether linkages, multi-dentate chelating ligand
- Selective chelation: High binding affinity for Ca2+, weak complexation with Mg2+, used to separate calcium/magnesium ions
- Acid-base property: Tetraprotic weak organic acid, reacts with NaOH, KOH to form soluble EGTA sodium salts
- Metal complexation: Forms stable water-soluble chelate complexes with alkaline earth, heavy metal ions
- Thermal stability: Stable below 200 ℃; high temperature heating causes decarboxylation degradation
- Photostability: Stable under normal light, no discoloration during sealed storage
- Compatibility: Compatible with neutral buffer solutions, biochemical reagents; incompatible with concentrated strong acid, strong oxidants
3. Main Application Fields
1. Biochemical Laboratory Reagent (Core Application)
- Calcium ion chelating agent for cell biology, enzyme activity experiments, calcium signal research
- Buffer additive for protein purification, intracellular calcium separation; selectively mask Ca without interfering Mg-dependent enzymes
- Metal ion masking agent for spectrophotometric and electrochemical analysis
2. Water Treatment & Analytical Chemistry
- Masking agent for determination of magnesium content in water samples by shielding calcium interference
- Standard chelating reagent for complexometric titration of calcium ion
3. Electroplating Industry Additive
Auxiliary complexing agent for nickel, zinc plating solution to stabilize metal ion concentration and smooth coating surface
4. Pharmaceutical Research Auxiliary
Metal ion stabilizer for in vitro drug metabolism and cell culture medium preparation
4. Quality Specification Table
| Test Item | Standard Index |
| CAS Number | 67-42-5 |
| Molecular Formula | C14H24N2O10 |
| Molecular Weight | 380.34 |
| Appearance | White crystalline powder |
| HPLC Purity | Biochemical Grade ≥99.0%; Analytical Grade ≥99.5% |
| Moisture Content (KF) | ≤0.30% |
| Residue on Ignition | ≤0.10% |
| Heavy Metals (Pb) | ≤10 ppm |
| Bulk Package | Aluminum foil vacuum bag / amber glass bottle |
| Storage Condition | Hermetic cool dry warehouse ≤25℃; isolate humid air and strong oxidants |
| Transportation Rule | Organic chelating solid, moisture-proof sealed packaging |
| Shelf Life | 24 months under intact vacuum packaging |
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