Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
1. Basic Information
Name: N-Hydroxysuccinimide
Abbreviation: HOSu, NHS
CAS No.: 6066-82-6
Molecular Formula: C4H5NO3
Letter-number format formula: C4H5N1O3
Molecular Weight: 115.09
2. Physical and Chemical Properties
Physical Properties
- Appearance: White crystalline powder
- Odor: Odorless
- Solubility: Soluble in water, methanol, ethanol, DMF, DMSO; slightly soluble in ethyl acetate; insoluble in hexane, benzene
- Melting Point: 95–98 °C
- Hygroscopicity: Slightly hygroscopic under humid conditions
Chemical Properties
- Molecular Structure: Five-membered succinimide ring with N-linked hydroxyl functional group
- Active ester synthesis reagent: Reacts with carboxylic acids under carbodiimide (EDC/DCC) to form NHS active esters, used for amide coupling
- Hydrolysis property: Stable in anhydrous environment; slowly hydrolyzes in water to succinimide and hydroxyl ions
- Thermal stability: Stable below 80 °C; decomposes above 120 °C to release irritant gas
- Photostability: Slightly sensitive to strong UV light; long-term light exposure turns pale yellow
- Compatibility: Compatible with anhydrous polar aprotic solvents; incompatible with strong oxidants, strong alkali, aldehydes
3. Main Application Fields
1. Peptide & Pharmaceutical Synthesis Reagent (Core Application)
- Amine coupling activator for solid-phase peptide synthesis, improves reaction yield and reduces racemization
- Raw material for manufacturing Boc/Fmoc protected amino acid active ester intermediates for APIs
- Cross-linking agent for antibody, protein bioconjugation in biopharmaceutical research
2. Biochemical Laboratory Reagent
- Protein labeling, enzyme immobilization and antibody modification auxiliary reagent
- Activator for preparation of fluorescent probes and small molecule bioconjugates
3. Fine Chemical Intermediate
Synthetic building block for amine-containing agrochemical and cosmetic functional additives
4. Reference Standard
HPLC calibration standard for succinimide active ester impurity testing in pharmaceutical production
4. Quality Specification Table
| Test Item | Standard Index |
| CAS Number | 6066-82-6 |
| Molecular Formula | C4H5N1O3 |
| Appearance | White crystalline powder |
| HPLC Purity | Peptide Grade ≥99.0%; Reference Standard ≥99.5% |
| Melting Point | 95–98 °C |
| Water Content (KF) | ≤0.20% |
| Residue on Ignition | ≤0.05% |
| Heavy Metals (Pb) | ≤10 ppm |
| Packaging | Nitrogen-filled amber aluminum foil vacuum bag / 25kg PE-lined fiber drum |
| Storage Condition | Hermetic cool dark warehouse below 25 °C; segregated from moisture, alkaline and oxidizing substances |
| Transportation Requirement | Moisture-sensitive coupling reagent solid, dust-proof light-resistant sealed packaging |
| Shelf Life | 24 months under intact nitrogen-sealed vacuum packaging |
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