Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
Polysucrose
1. Basic Information
Name: Polysucrose
Alias: Ficoll, Ficoll 400, Sucrose Epichlorohydrin Copolymer
CAS No.: 26873-85-8
Molecular Formula: Polymer, no fixed single molecular formula, average molecular weight ~400,000 Da
Category: Neutral hydrophilic polysaccharide polymer, biological separation medium, biochemical reagent
2. Physical and Chemical Properties
Physical Properties
- Appearance: White to off-white fine amorphous powder
- Odor: Odorless
- Solubility: Highly soluble in water, phosphate buffer, saline solution; slightly soluble in DMSO; insoluble in ethanol, acetone, ethyl acetate, alkanes
- Melting characteristic: No clear melting point, decomposes above 280 °C
- Hygroscopy: Strongly hygroscopic, quickly absorbs moisture in air
- Aqueous property: Neutral aqueous solution, pH 6.0–7.5, low viscosity at low concentration, high viscosity under high concentration
Chemical Properties
- Molecular structure: Cross-linked polymer formed by sucrose polymerized with epichlorohydrin, rich in hydroxyl groups, neutral non-ionic macromolecule
- Hydroxyl reactivity: Surface hydroxyl groups can be modified for derivatization; no charge under physiological pH
- Biocompatibility: Non-toxic to cells, low protein adsorption, does not penetrate cell membrane
- Thermal stability: Stable below 120 °C; autoclave sterilization (121 °C) short time without obvious degradation; long-term high temperature causes molecular chain breakage
- Photostability: Stable under normal light; long-term strong UV irradiation leads to slight molecular degradation
- Compatibility: Compatible with common biological buffers, inorganic salts; incompatible with strong oxidants, concentrated strong acid/alkali
3. Main Application Fields
1. Cell Density Gradient Separation (Core Application)
Widely used in immunology, hematology for separating peripheral blood mononuclear cells, lymphocytes, monocytes; configure isotonic density gradient medium for cell isolation
2. Biochemical & Biophysics Research
Macromolecular crowding agent simulating intracellular environment; molecular sieve auxiliary for gel filtration; density adjuster for organelle separation
3. Pharmaceutical Excipient
Stabilizer, cryoprotectant for protein, antibody, vaccine formulations
4. Laboratory Calibration Standard
Molecular weight marker for aqueous phase size-exclusion chromatography
4. Quality Specification Table
| Test Item | Standard Specification |
| CAS Number | 26873-85-8 |
| Average Molecular Weight | ~400,000 |
| Appearance | Uniform white powder without agglomeration |
| Water Content (KF) | ≤5.0% |
| Heavy Metals (Pb) | ≤10 ppm |
| Endotoxin | ≤0.1 EU/mg (cell separation grade) |
| Residue on Ignition | ≤0.20% |
| Packaging | Nitrogen-sealed PE bottle, vacuum aluminum foil bag inner packaging |
| Storage Condition | Hermetically sealed cool dry environment below 25 °C; avoid moisture absorption, separate from strong acid and oxidants |
| Transportation Requirement | Hygroscopic biochemical polymer solid, moisture-proof sealed packaging |
| Shelf Life | 36 months under intact sealed storage |
Safety Warning
Low-toxicity mild irritant polysaccharide powder. Dust may irritate eyes and respiratory tract; long-term skin contact causes dryness. All operations shall be carried out in ventilated space with dust mask and disposable gloves. Avoid high-temperature burning which releases carbohydrate decomposition fumes. Waste aqueous solution can be diluted and discharged after neutralization; solid waste belongs to general biochemical waste and disposed by licensed hazardous waste institutions.
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