Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
Hematoxylin
1. Basic Information
Name: Hematoxylin
Alias: Hematoxyline, Natural Black Stain, 7,11b-Dihydroxy-3,4,6,11b-tetrahydro-1H,10H-benzo[b]pyrano[3,2-g]chromen-1-one
CAS No.: 517-28-2
Molecular Formula: C16H14O6
Molecular Weight: 302.28
Category: Natural plant extract histology staining reagent, biological dye raw material, biochemical laboratory stain
2. Physical and Chemical Properties
Physical Properties
- Appearance: Pale yellow to off-white crystalline powder, gradually darkens to reddish-brown under air and light
- Odor: Odorless
- Solubility: Slightly soluble in cold water, soluble in hot water, ethanol, methanol, glycerol, alkaline aqueous solution; insoluble in petroleum ether, benzene
- Melting Point: 140–145 ℃ (decomposes)
- Density (predicted): 1.48 g/cm3
- Hygroscopy: Slightly hygroscopic under high humidity
- Aqueous property: Multiple phenolic hydroxyl groups, weak acidity; dissolves in alkali to form deep purple-red solution
Chemical Properties
- Molecular structure: Natural polyphenol fused bicyclic chroman skeleton with five phenolic hydroxyl groups, strong color-developing activity
- Oxidation color reaction: Raw hematoxylin is colorless; oxidized to hematein to form blue-purple stain which combines with metal mordants (aluminum, iron) for nuclear staining
- Metal complexation: Forms stable colored chelates with Al3?, Fe3?; core staining core mechanism in histopathology
- Thermal stability: Stable below 100 ℃; heating above melting point causes oxidative decomposition and color loss
- Photostability: Extremely light-sensitive; UV/long-term sunlight exposure accelerates oxidation and darkens powder
- Compatibility: Compatible with alcohol, glycerol, weak alkaline buffer; incompatible with strong oxidants, concentrated strong acid
3. Main Application Fields
1. Histopathology & Cytology Stain (Core Application)
Main nuclear staining reagent for biological tissue sections, cell smears; matched with eosin to prepare H&E staining solution for medical pathology laboratory
2. Biochemical Research Reagent
Morphological observation stain for cell culture, microorganism tissue slice analysis
3. Natural Dye Raw Material
Plant-based mordant dye for textile, leather color finishing
4. Standard Reference Substance
Color standard for histological stain quality inspection and laboratory reagent calibration
4. Quality Specification Table
| Test Item | Standard Specification |
| CAS Number | 517-28-2 |
| Molecular Formula | C16H14O6 |
| Appearance | Pale yellow crystal powder without black oxidized impurities |
| HPLC Purity | Histology Grade ≥98.0% |
| Water Content (KF) | ≤0.50% |
| Residue on Ignition | ≤0.10% |
| Staining Activity | Qualified for nucleus clear staining |
| Heavy Metals (Pb) | ≤10 ppm |
| Packaging | 10g/100g amber glass bottle, 1kg/5kg vacuum aluminum foil bag, 25kg light-proof fiber drum |
| Storage Condition | Hermetically sealed cool dry warehouse 2–8 ℃, full light shielding, isolate air and oxidizing substances |
| Transportation Requirement | Highly light-sensitive natural polyphenol powder, fully sealed light-proof moisture-proof packaging |
| Shelf Life | 24 months under nitrogen vacuum sealed low-temperature storage |
Safety Warning
Low-toxic mild irritant plant extract powder. Dust irritates eyes, nasal tract and respiratory mucosa; long-term skin contact may cause temporary pigment staining. All operations carried out in ventilated fume hood with dust-proof respirator and nitrile gloves. Avoid mixing with strong oxidants to prevent rapid discoloration and failure of staining performance. Waste liquid neutralized before discharge; solid waste classified as general biological laboratory waste for professional disposal.
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