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1,3-Bis[tris(hydroxymethyl)methylamino]propane
1. Basic Information
Name: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
Alias: BIS-TRIS propane
CAS No.: 64431-96-5
Molecular Formula: C11H26N2O6
Molecular Weight: 282.33
Category: Biological buffer reagent, cell culture pH stabilizer, biochemical laboratory raw material, electrophoresis buffer additive
2. Physical and Chemical Properties
Physical Properties
- Appearance: White crystalline powder
- Odor: Odorless
- Solubility: Freely soluble in water; soluble in methanol; slightly soluble in ethanol; insoluble in acetone, ether, hydrocarbon solvents
- Melting Point: 164–168 ℃
- Density (predicted): 1.23 g/cm3
- Hygroscopy: Moderately hygroscopic, easy to absorb moisture under high humidity
- Aqueous property: Weak alkaline buffer, effective buffering range pH 6.0–9.0; stable aqueous solution at room temperature
Chemical Properties
- Molecular structure: Two tris(hydroxymethyl)aminomethyl groups connected by propane alkyl chain, multiple hydroxyl groups and tertiary amine groups
- Buffer performance: Excellent pH buffering capacity, low metal ion chelation interference, low cytotoxicity
- Hydroxyl characteristic: Multi-hydroxyl structure has weak metal chelating ability, does not interfere with most enzyme activity detection
- Thermal stability: Stable below 120 ℃; long-time high temperature heating will cause slight dehydration
- Photostability: Good light resistance, no discoloration under long-term light irradiation
- Compatibility: Compatible with various cell culture mediums, biochemical detection reagents; incompatible with strong oxidants, concentrated strong acid
3. Main Application Fields
1. Biochemical Buffer Reagent (Core Application)
Universal biological buffer for protein purification, enzyme activity assay, cell culture; replace phosphate buffer to avoid metal ion precipitation interference
2. Electrophoresis & Chromatography Auxiliary
pH stabilizer for protein electrophoresis, ion chromatography mobile phase additive
3. Cell & Microorganism Culture Medium
Low-toxicity pH regulator for mammalian cell, yeast fermentation culture system
4. Medical Diagnostic Reagent Raw Material
Buffer component for in vitro diagnostic kits, immune detection reagent
4. Quality Specification Table
| Test Item | Standard Specification |
| CAS Number | 64431-96-5 |
| Molecular Formula | C11H26N2O6 |
| Appearance | Uniform white crystal powder without yellowing |
| HPLC Purity | Biochemical Reagent Grade ≥99.0% |
| Water Content (KF) | ≤1.00% |
| Residue on Ignition | ≤0.10% |
| pH (1% aqueous solution) | 7.0–8.0 |
| Heavy Metals (Pb) | ≤5 ppm |
| Microbial Limit | Total bacteria ≤100 CFU/g, no pathogenic bacteria |
| Packaging | Nitrogen-filled aluminum foil vacuum bag; bulk 1kg/5kg PE lined fiber drum |
| Storage Condition | Hermetically sealed cool dry warehouse below 25 ℃, isolate humid air and strong oxidants |
| Transportation Requirement | Slightly hygroscopic biochemical buffer powder, moisture-proof sealed packaging |
| Shelf Life | 24 months under nitrogen vacuum sealed storage |
Safety Warning
Low irritation polyhydroxy amine powder. Dust slightly irritates eyes and respiratory tract. All operations in ventilated environment with dust mask and nitrile gloves. Waste liquid can be diluted and discharged normally; solid waste belongs to general biochemical reagent waste. Low acute toxicity, safe for laboratory conventional use.
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