Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
Hygromycin B
1. Basic Information
Name: Hygromycin B
Alias: O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1→2)-O-β-D-talopyranosyl-(1→5)-2-deoxy-N3-methyl-D-streptamine
CAS No.: 31282-04-9
Molecular Formula: C20H37N3O13
Molecular Weight: 523.52
Category: Aminoglycoside antibiotic, cell screening selective agent, genetic engineering screening reagent, microbial fermentation inhibitor
2. Physical and Chemical Properties
Physical Properties
- Appearance: White to off-white amorphous lyophilized powder
- Odor: Odorless, slight bitter taste
- Solubility: Freely soluble in water, phosphate buffer; soluble in methanol; slightly soluble in ethanol; insoluble in acetone, ethyl acetate, hydrocarbon solvents
- Melting Point: Decomposes above 190 ℃, no distinct melting point
- Density (predicted): 1.45 g/cm3
- Hygroscopy: Strongly hygroscopic, easily absorbs moisture and agglomerates under high humidity
- Aqueous property: Weakly acidic aqueous solution, pH 4.0–6.0; stable in acidic environment, rapidly deactivated under strong alkaline heating
Chemical Properties
- Molecular structure: Polyhydroxy aminoglycoside trisaccharide structure, multiple amino groups and hydroxyl groups; inhibits prokaryotic and eukaryotic ribosomal protein synthesis
- Antibacterial & screening mechanism: Blocks ribosomal translocation step, kills bacteria, yeast, fungi and mammalian cells without resistance cross-reaction with neomycin, kanamycin
- Alkaline hydrolysis sensitivity: Strong alkali breaks glycosidic bonds, decomposes into sugar fragments and loses biological activity
- Oxidation degradation: Heavy metal ions, UV light accelerate oxidation of amino groups to form inactive impurities
- Thermal stability: Stable below 40 ℃; boiling or high-temperature sterilization leads to irreversible loss of activity
- Photostability: Light-sensitive; long-term light exposure reduces titer significantly
- Compatibility: Compatible with neutral/weak acidic culture medium, mannitol, trehalose; incompatible with strong alkali, oxidants, copper/iron heavy metal salts
3. Main Application Fields
1. Genetic Engineering Cell Screening Reagent (Core Application)
Selective antibiotic for eukaryotic cell, yeast, plant cell screening; matched with hygromycin resistance gene (hph) to screen stably transfected cell lines
2. Microbial Culture Inhibitor
Suppresses mixed bacteria contamination in fermentation of fungi and actinomycetes
3. Molecular Biology Laboratory Standard Reagent
Positive control for antibiotic resistance gene verification, cell death induction experimental material
4. Agricultural Antimicrobial Raw Material
Low-dose additive to inhibit plant pathogenic fungi in tissue culture seedling medium
4. Quality Specification Table
| Test Item | Standard Specification |
| CAS Number | 31282-04-9 |
| Molecular Formula | C20H37N3O13 |
| Appearance | Uniform white lyophilized powder without yellow discoloration |
| HPLC Potency | Cell Culture Grade ≥980 U/mg, High Purity Reagent Grade ≥99.0% |
| Water Content (KF) | ≤3.00% |
| Residue on Ignition | ≤0.50% |
| Single Related Impurity | ≤0.30% |
| Total Impurities | ≤1.20% |
| Heavy Metals (Pb) | ≤5 ppm |
| Microbial Limit | Sterile raw material, no bacteria, fungi or mycoplasma |
| Packaging | Nitrogen-filled amber light-proof glass vial; bulk 1kg/5kg light-proof PE lined freeze-drying fiber drum |
| Storage Condition | Hermetically sealed constant temperature warehouse 2–8 ℃, full light shielding, isolate humid air and alkaline raw materials |
| Transportation Requirement | Light-sensitive strongly hygroscopic biological antibiotic powder, cold-chain sealed light-blocking packaging, avoid high temperature and sunlight |
| Shelf Life | 36 months under nitrogen vacuum sealed refrigerated storage |
Safety Warning
Allergenic irritant antibiotic powder. Dust inhalation easily triggers respiratory allergy, cough and chest tightness; eye/mucous contact causes severe irritation, skin contact may induce allergic dermatitis. All operations must be carried out in light-proof sterile fume hood with biological dust respirator, UV-blocking splash goggles and thick peptide-resistant nitrile gloves. Strictly avoid light, high temperature and contact with alkaline substances. Waste liquid treated by high-temperature inactivation before discharge; solid waste classified as biological antibiotic hazardous waste for professional incineration disposal. Operators with antibiotic allergies must avoid all contact.
...
Product
