Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
Methotrexate
1. Basic Product Information
Product Name: Methotrexate
CAS Number: 59-05-2
Molecular Formula: C20H22N8O5
Molecular Weight: 454.44
Synonyms: Amethopterin, 2,4-Diamino-N10-methylpteroylglutamic acid
Product Category: Anti-tumor pharmaceutical API, immunosuppressant bulk drug, folate antagonist biochemical reagent
2. Physical and Chemical Properties
Physical Indicators
- Appearance: Yellow to orange crystalline powder
- Odor: Odorless
- Solubility: Slightly soluble in dilute hydrochloric acid; soluble in dilute alkaline aqueous solution; barely soluble in water, ethanol, chloroform and ether
- Melting Point: Decomposes at 185–204 °C
- Density (20 °C): 1.46 g/cm3
- Hygroscopicity: Moderately hygroscopic under high humidity conditions
- Aqueous Stability: Stable under weak alkaline environment; rapidly degraded under strong acid, high temperature and strong ultraviolet light
Chemical Characteristics
- Molecular Structure: Pteridine heterocyclic ring linked with methylamino benzoyl and glutamic acid side chain, multi-nitrogen folic acid analog structure
- Pharmacological Mechanism: Competitive inhibitor of dihydrofolate reductase, blocks DNA and RNA synthesis to suppress tumor cell proliferation and abnormal immune cell activity
- Photodegradation Property: Extremely light-sensitive; UV irradiation triggers ring-opening degradation and color fading, leading to loss of biological activity
- Thermal Stability: Stable below 60 °C; irreversible decomposition occurs above 150 °C, releasing irritating nitrogen-containing heterocyclic fumes
- Chemical Compatibility: Compatible with sterile weak alkaline buffer solutions; incompatible with strong acids, heavy metal ions, strong oxidants and reducing agents
3. Main Application Fields
- Core Application: Human Anti-Cancer Injectable & Oral API
Approved therapeutic raw material for chemotherapy of various malignant tumors including leukemia, breast cancer, osteosarcoma; also used for autoimmune disease treatment such as rheumatoid arthritis and psoriasis - Pharmaceutical Formulation Intermediate
Raw material for methotrexate tablets, lyophilized injection and subcutaneous injection preparations - Biomedical Laboratory Research Reagent
Standard folate antagonist for tumor cell culture, immunology and cell metabolism research - HPLC Certified Reference Standard
Control substance for related impurity testing of methotrexate bulk drug and finished pharmaceutical products
4. Platform Category & Product Group Selection Guide
1) Domestic B2B Platform Category
Recommended Path: Organic Intermediates / Pharmaceutical Intermediates
Reason: Regulated prescription anti-tumor human pharmaceutical active ingredient, classified as pharmaceutical intermediate
2) English Product Group Dropdown Options
Recommended Selection: Pharmaceutical Chemicals
Do NOT select Basic Organic Chemicals; this is controlled therapeutic pharmaceutical raw material matching pharmaceutical chemical grouping
3. Standard Foreign Trade Packaging Specification
5mg/10mg nitrogen-sealed glass vials for lab research samples; 1g/5g aluminum foil vacuum bags, 25kg double PE-lined fiber drums for bulk sterile API orders; customized pharmaceutical-grade low-temperature packaging available
5. Quality Specification Table
| Test Item | Standard Requirement |
| CAS Number | 59-05-2 |
| Molecular Formula | C20H22N8O5 |
| Appearance | Uniform orange crystalline powder without pale or dark discoloration |
| HPLC Purity | Pharma API Grade ≥98.5%, Reference Standard ≥99.5% |
| Water Content (KF Method) | ≤0.50% |
| Single Unknown Impurity | ≤0.10% |
| Total Related Substances | ≤0.50% |
| Heavy Metals (Pb) | ≤10 ppm |
| Bacterial Endotoxin | ≤0.1 EU/mg (Injection Grade) |
| Standard Storage Package | Light-proof nitrogen-filled aluminum foil vacuum bag, low-temperature sealed container |
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