Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
DL-1,4-Dithiothreitol
1. Basic Product Information
Product Name: DL-1,4-Dithiothreitol
Abbreviation: DTT
CAS Number: 3483-12-3
Molecular Formula: C4H10O2S2
Molecular Weight: 154.25
Synonyms: Cleland's reagent, DL-Dithiothreitol, 2,3-Dihydroxy-1,4-butanedithiol
Product Category: Biochemical reducing agent, protein research reagent, peptide synthesis auxiliary, laboratory analytical chemical
2. Physical and Chemical Properties
Physical Indicators
- Appearance: White crystalline powder
- Odor: Faint sulfurous mercaptan odor
- Solubility: Freely soluble in water, ethanol, methanol; slightly soluble in acetone; insoluble in ether, benzene and alkanes
- Melting Point: 42–44 °C
- Density (20 °C): 1.26 g/cm3
- Hygroscopicity: Moderately hygroscopic, absorbs moisture rapidly under high humidity
- Aqueous Stability: Aqueous solution oxidizes quickly when exposed to air; stable under nitrogen protection and weak acidic environment
Chemical Characteristics
- Molecular Structure: Four-carbon straight chain with two hydroxyl groups and two thiol (-SH) groups at both terminals
- Core Functional Mechanism: Powerful disulfide bond reducing agent; breaks S-S bonds in proteins and peptides to maintain cysteine sulfhydryl activity; forms stable six-membered cyclic disulfide after oxidation
- Oxidation Sensitivity: Extremely susceptible to oxidation by oxygen in air, especially in neutral/alkaline aqueous solution, losing reducing activity
- Photostability: Strongly light-sensitive; UV irradiation accelerates thiol oxidation and deactivation
- Thermal Stability: Stable below 35 °C; above melting point, thiol groups volatilize and oxidize rapidly
- Chemical Compatibility: Compatible with neutral and weak acidic biological buffers; incompatible with air, strong oxidants, heavy metal ions and alkaline solutions
3. Main Application Fields
- Core Application: Biochemistry & Molecular Biology Lab Reagent
Reducing agent for protein denaturation, SDS-PAGE electrophoresis, enzyme activity protection, antibody purification and peptide unfolding; widely used in cell biology and protein research - Peptide & Nucleic Acid Synthesis Auxiliary
Protects free sulfhydryl groups during solid-phase peptide synthesis and DNA/RNA extraction - Immunology Experimental Reagent
Additive for antigen-antibody incubation systems to prevent protein disulfide cross-linking and maintain antigen activity - Chiral Synthetic Reducing Substrate
Reducing auxiliary for chiral sulfide and natural product total synthesis
4. Platform Category & Product Group Selection Guide
1) Domestic B2B Platform Category
Recommended Path: Organic Intermediates / Organic Intermediates
Reason: Specialized biochemical thiol reagent for laboratory protein research
2) English Product Group Dropdown Options
Recommended Selection: Basic Organic Chemicals
Do NOT select Pharmaceutical Chemicals; it is laboratory biochemical reagent instead of human drug bulk raw material
3. Standard Foreign Trade Packaging Specification
1g/5g nitrogen-sealed glass vials for lab micro-experiments; 1kg aluminum foil vacuum bag for biotech factories; 25kg double PE lined fiber drum with nitrogen filling for bulk orders
5. Quality Specification Table
| Test Item | Standard Requirement |
| CAS Number | 3483-12-3 |
| Molecular Formula | C4H10O2S2 |
| Appearance | Uniform white crystalline powder without yellow oxidized impurities |
| HPLC Purity | Biochemical Grade ≥99.0%, Ultra-Pure Research Grade ≥99.5% |
| Melting Point | 42–44 °C |
| Water Content (KF Method) | ≤0.30% |
| Oxidized DTT Impurity | ≤0.10% |
| Total Related Substances | ≤0.40% |
| Heavy Metals (Pb) | ≤10 ppm |
| Standard Storage Package | Light-proof nitrogen-filled vacuum aluminum foil bag, low-temperature sealed container |
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