Shandong Huashang Chemical Co., Ltd. is a high-tech enterprise focused on industry of fine chemicals research and develop, produce and sale. Shandong Huashang Chemical Co., Ltd. is located in Shan Dong Ji Nan China. we have the ability to provide you high quality products. More than 90 % of our products are exported to developed countries and regions such as north America, Europe, Japan, South Korea and the Middle East.
HEPES
1. Basic Product Information
Product Name: HEPES
Full Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
CAS Number: 7365-45-9
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.30
Synonyms: 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
Product Category: Biological buffer reagent, cell culture medium raw material, biochemical analytical auxiliary, pharmaceutical excipient
2. Physical and Chemical Properties
Physical Indicators
- Appearance: White crystalline powder
- Odor: Odorless
- Solubility: Freely soluble in water (500 mg/mL); insoluble in acetone, ether, benzene and alkane solvents
- Melting Point: 234–238 °C (decomposes)
- pKa (25°C): 7.5
- Effective buffering pH range: 6.8–8.2
- Density (20 °C): 1.07 g/cm3
- Hygroscopicity: Slightly hygroscopic under high humidity environment
- Aqueous Stability: Stable under neutral and weakly acidic conditions; maintains stable pH independent of CO? balance
Chemical Characteristics
- Molecular Structure: Zwitterionic Good’s buffer containing piperazine ring, hydroxyl group and sulfonic acid group
- Core Functional Mechanism: Physiological pH stabilizer with weak metal chelating ability, low UV absorbance, no interference with most enzyme and immunoassays
- Photostability: Excellent light stability under sealed dry storage
- Thermal Stability: Stable below 200 °C; thermal decomposition above melting point releases irritant sulfonic acid fumes
- Chemical Compatibility: Compatible with cell culture media, inorganic salts and neutral biological buffers; incompatible with strong oxidants and concentrated strong alkalis
3. Main Application Fields
- Core Application: Cell & Tissue Culture Buffer
Primary pH stabilizer for mammalian cell culture medium; maintains steady pH without CO? incubator and prevents pH fluctuation caused by CO? volatilization - Biochemical & Molecular Biology Reagent
Buffer for enzyme activity detection, protein electrophoresis, antigen-antibody incubation and isoelectric focusing electrophoresis; not suitable for Folin-Ciocalteu protein quantification - Immunology & Drug Screening Auxiliary
Stable buffer for receptor binding assays and in vitro cell drug stimulation tests - Pharmaceutical Excipient
pH regulator and stabilizer for injection solutions and topical biological ointments
4. Platform Category & Product Group Guidance
1) Domestic B2B Platform Category
Recommended Path: Organic Intermediates / Biochemical Reagents
Reason: Special zwitterionic biological buffer for laboratory cell and protein research
2) English Product Group Dropdown Options
Recommended Selection: Basic Organic Chemicals
Do NOT select Pharmaceutical Chemicals exclusively; it is a general laboratory biochemical reagent
3. Standard Foreign Trade Packaging Specification
10g / 100g sealed glass bottles for small lab experiments; 1kg aluminum foil vacuum bags for biotech manufacturers; 25kg double PE-lined fiber drums for bulk orders
5. Quality Specification Table
| Test Item | Standard Requirement |
| CAS Number | 7365-45-9 |
| Molecular Formula | C8H18N2O4S |
| Appearance | Uniform white crystalline powder free of yellow impurities |
| Titration Purity | Cell Culture Grade ≥99.5%, Reagent Grade ≥99.0% |
| Water Content (KF Method) | ≤0.30% |
| Heavy Metals (Pb) | ≤10 ppm |
| Absorbance (260nm, 0.1M) | ≤0.02 |
| pH (238g/L aqueous solution) | 5.0–6.5 |
| Standard Storage Package | Lightproof sealed aluminum foil bag, store in dry warehouse |
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